|
1 / 1
|
DeCS
|
|
 |
|
Descriptor English:
|
|
Freeze Etching
|
|
Descriptor Spanish:
|
|
Grabado por Congelación
|
|
Descriptor Portuguese:
|
|
Técnica de Congelamento e Réplica
|
|
Synonyms English:
|
|
Etching, Freeze
|
|
Tree Number:
|
|
E01.370.225.500.620.620.260.400
E01.370.225.750.600.620.260.400
E05.200.500.620.620.260.400
E05.200.750.600.620.260.400
|
|
Definition English:
|
|
A replica technique in which cells are frozen to a very low temperature and cracked with a knife blade to expose the interior surfaces of the cells or cell membranes. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the sublimation of water during the freeze-drying process to etch the surfaces. |
|
Indexing Annotation English:
|
|
do not confuse with FREEZE FRACTURING (wherein a cryoprotectant is used)
|
|
History Note English:
|
|
73(69)
|
|
Allowable Qualifiers English:
|
|
|
|
Record Number:
|
|
5749
|
|
Unique Identifier:
|
|
D005613
|
Occurrence in VHL:
|
|
|
Similar:
|
|
DeCS
|